Hasil Penelitian Dosen
DEVELOPING NATRIUM FLUORIDE PATCH WITH ENHANCER IN DENTISTRY: IN VITRO AND IN VIVO TRANSDERMAL DELIVERY
- Bagikan:
Fluoride plays an important roles in reducing dental caries by improving
remineralization and strengthening email through fluoroapatite forming which is more
resistant to acid. The available fluoride dosage form have disadvantages due to its
difficulties to control dose and to overcome first hepatic metabolism. Fluoride systemic
delivery system to maintain therapeutic plasma concentration need to be developed, and one
attractive candidate is transdermal route. Aims this research were to develop patch
containing natrium fluoride, to find fluoride concentration on blood and teeth after
application and also to study its biocompatibility.
Quasy experimental design with post test only control group as research approach
was used. Serial transport test with vertical diffusion cell was used as the instrument to
examine in vitro skin permeation test with full thickness rat skin as membrane between
donor and receptor compartment. Donor was filled with either fluoride solution, fluoride
added chemical enhancer and fluoride patch. Sample was taken for time interval of 8, 24
and 48 hours and fluoride concentration was measured by Potensiometer Spesific Fluoride
Ion. An in vivo experimentation was done by appliying NaF patch onto dorsal mouse skin
and blood sample was taken after 8, 24 and 48 hours to determine fluoride concentration.
Teeth was extracted two weeks after application to determine fluoride. Treatment group was
patch containing NaF 1.000, 750 and 500 ppm, while patch without NaF was used in control
group. Skin sensitisation was evaluated visually for biocompatibility test.
Research results showed fluoride can penetrate to the skin. Time of transport
influences weight of fluoride and enhancer can increase absorption rate of fluoride. Fluoride
weight after transport test of NaF patch for 8-48 hours with patch concentration 0, 500, 750
dan 1.000 mg/L (ppm) was 0.06-0.18 μg; 0.33-0.94 μg; 0.72-1.23 μg; 0.88-1.73 μg. In vivo
experiment showed fluoride concentration in blood plasma after application of NaF patch
for 0, 8, 24 and 48 hours were 3.9-4.11 ppb; 4.46-9.5 ppb; 4.18-13.4 ppb and 3.9-5.6 ppb
respectively. Repeated Anova showed significantly differences fluoride concentration based
on patch fluoride concentration and time of application (p
Informasi Detail
| Pernyataan Tanggungjawab | |
|---|---|
| Pengarang |
Diyah Fatmasari - Pengarang Utama
|
| NIM | |
| Bahasa |
English
|
| Deskripsi Fisik |
14 hal, illus., 23 cm
|
| Dilihat sebanyak |
668
|
| Penerbit | : Semarang., 2017 |
|---|---|
| Edisi | |
| Subjek | |
| Klasifikasi |
617.9
|